Coding

Part:BBa_K4606004

Designed by: Baochao Li   Group: iGEM23_Nanjing-NFLS   (2023-09-14)


SMAD4(mutant type)

Description

For detailed design information, you can look up the registry of BBa_K4606003, the device for gene segment of SMAD4.

According to the base sequence information of SMAD4 in the database, we mutated the SMAD4 gene in a region that complementary binds to miR-3074-5p. By inserting its 3’UTR region (414bp) behind the luciferase gene in the vector, we could determine the expression level of this gene segment.

Figure3 (Part4). Acquisition of SMAD4-MUT gene segments by PCR.
1#: DNA marker. 2#: Segments of target gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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